Whole Genome Sequencing

Whole Genome Sequencing

Our center utilizes Illumina DNA library preparations to generate consistently, high quality libraries from a wide range of sample types.  All samples are processed in the same manner and sequenced to the requested depth on the NextSeq 2000 platform.  

This service will provide paired end reads (2x151bp) delivered as fastq files.  The total amount of reads you receive will be based upon the following list of sequencing depths available:

Sequencing Package (Guaranteed minimum number of >Q30 base pair reads)*	Example Uses	Price**
200Mbp	Plasmids, Viral dsDNA, bacterial genomes <5Mb	$80.00
400Mbp	Bacterial genomes requiring extra coverage or >5Mb in size	$105.00
650Mbp	Bacterial populations, yeast genomes	$140.00
1Gbp	Large yeast genomes, metagenomes, small algae	$200.00
2Gbp	Metagenomes, small algae	$320.00

*Average delivered amount is approximately 2-3 times higher
**Orders >48 Samples receive 10% off using Discount Code: Bulk10Savings

Sample Requirements:

• 30uL or more total volume
• Sample concentration of greater than 10ng/uL measured by Qubit*** (or 20ng/uL measured by Nanordrop)
• Eluted into water or dilute Tris/TE buffer
• Fragments shorter than 400bp are unable to be processed

Additional Options

• Genomic DNA isolation is available at a rate of $15 per sample 
  • Pre-grown agar plates and frozen cell pellets (10^9 cells recommended) are accepted
  • For Bacterial Samples: Only strains amenable to lysis by lysozyme/Proteinase K treatment are able to be extracted
  • For Yeast Samples: Only strains amenable to Zymolyase digestion followed by Proteinase K treatment are able to be extracted
  • Staph samples will be accepted if they are sent with an aliquot of lysophilized Lysostaphin
  • We do not accept live BSL3 or above samples for extractions
• Variant Calling Analysis is available at a rate of $10 per sample
  • Only applicable to haploid genomes (for other genomes, please contact us)
  • Assembled reference genome must be provided (.dna and .snap formats are not accepted)

Our facility provides long reads using the Oxford Nanopore platform. We use a PCR-free ligation library preparation that provides reads equal to the length of the input DNA, which have been shown to assemble into complete genomes with as little as 12x coverage.  Long reads are also an excellent tool for sorting out highly repetitive regions of genomes, determining the location of mobile genetic elements and methylation studies.

Offering	Description	Price
300Mbp	Minimum of 300Mbp Long Reads Only	$350.00
900Mbp	Minimum of 900Mbp Long Reads Only	$550.00

When used in combination with Illumina reads (to improve accuracy at the nucleotide level), long read sequencing is a perfect choice for closing genomes and assembling communities.  Our facility uses the latest, proven software to combine the two data types into a single, annotated genome file.  Samples sequenced under these packages will receive both the ONT and Illumina raw reads, as well as the assembled and annotated genome in .fasta and .gff format.
 

Sequencing Package	Description	Price
Small Nanopore Combo	Minimum 300Mbp Long Reads, 400Mbp Illumina Reads, Genome Assembly and Annotation Ideal for Bacterial Genomes <8Mb	$500.00
Large Nanopore Combo	Minimum 900Mbp Long Reads, 1Gbp Illumina Reads, Genome Assembly and Annotation Ideal for Large Bacterial Genomes and Fungal Genomes <15Mb	$800.00

Sample Requirements

• Volume of 60uL or more 
  • For Nanopore Combo packages, please include a second, separate aliquot of gDNA for Illumina Sequencing (see above for requirements)
• Sample concentration greater than 40ng/uL (measured by Qubit), or greater than 100ng/uL (measured by Nanodrop)***

***Please see our FAQ for more information about measurement methods.