Whole Genome Sequencing
Our center utilizes a single library preparation method based upon the Illumina Nextera kit series. All samples are processed in the same manner and sequenced to the requested depth on the NextSeq 550 platform.
This service will provide paired end reads (2x150bp) delivered as fastq files. You will also receive files containing the index reads should you need them for custom filtering applications. The total amount of reads you receive will be based upon the following list of sequencing depths available:
(Guaranteed minimum number of >Q30 reads)*
|150Mbase||Plasmids, Viral dsDNA, bacterial genomes <5Mb||$80.00|
|300Mbase||Bacterial genomes requiring extra coverage or >5Mb in size||$100.00|
|625Mbase||Bacterial populations, yeast genomes||$125.00|
|1Gbase||Large yeast genomes, metagenomes, small algae||$200.00|
Our facility provides long reads using the Oxford Nanopore platform. We use a PCR-free ligation library preparation that provides reads equal to the length of the input DNA, which have been shown to assemble into complete genomes with as little as 12x coverage. Long reads are also an excellent tool for sorting out highly repetitive regions of genomes or determining the location of mobile genetic elements.
When used in combination with Illumina reads (to improve accuracy at the nucleotide level), long read sequencing is a perfect choice for closing genomes and assembling communities. Our facility uses the latest, proven software to combine the two data types into a single, annotated genome file.
|Nanopore 1||Minimum of 350Mbase Long Reads||$400.00|
|Nanopore 2||De novo assembly and annotation of your sample using Nanopore and Illumina reads
(Nanopore 1 and 300Mb Illumina sequencing are included)
*For genomes larger than 10Mb, please contact for pricing*
-50uL or more total volume
-Sample concentration greater than 50ng/uL (measured by Qubit), or greater than 100ng/uL (measured by Nanodrop)