Microbiome Sequencing

Our goal as a sequencing provider is to tailor existing technologies and sample preparation methods to better accommodate small genomes. By focusing our offerings on only the most highly demanded services, we are able to consistently provide a combination of price and turnaround time that is unmatched. All prices are fully inclusive of QC, library prep, sequencing and data distribution.

16S Sequencing

Our center utilizes Zymo Quick-16S Library Prep methods to selective amplify either the V3/V4 region (bacteria/archaea) or ITS2 region of your metagenomic sample.  This service will provide paired end reads (2x301bp) delivered as fastq files and preliminary analysis reports (no additional charge) to include the following:

  • Species Abundance Tables
  • Alpha and Beta Diversity
  • Taxonomic Assignment
  • Diversity Barplots by Taxonomic Rank

The total amount of reads you receive will be based upon the following list of sequencing depths available:

Sequencing Package* Price**
20k 16S Reads (10k Read Pairs, Either V3/V4 or ITS2 Region) $50.00
50k 16S Reads (25k Read Pairs, Either V3/V4 or ITS2 Region) $60.00
100k 16S Reads (50k Read Pairs, Either V3/V4 or ITS2 Region) $80.00

*Average delivered amount is approximately 2 times above the listed amount
** Orders >48 Samples receive 10% off using Discount Code: Bulk10Savings

Sample Requirements:

  • 20uL or more total volume
  • Sample concentration of greater than 10ng/uL of DNA measured by Qubit***
  • Eluted into water or dilute Tris/TE buffer
Additional Options
  • Genomic DNA isolation is available at a rate of $15 per sample.  We use Zymo Quick-DNA Fecal/Soil extractions which are compatible with a wide range of sample types.

Shotgun Metagenomics

Rather than target the the 16S gene region alone, our shotgun metagenomics approach sequences your sample's entire community using Illumina DNA Library Prep methods.  Community profiling is beneficial for users that are more interested in the functional gene content of their sample as opposed to the identification of the microbial genus and species that make it up.

  • Metagenomic Assembly
  • Assembly Annotation
  • Taxonomic Assignment 
The total amount of reads you receive will be based on the below list of sequencing depths available:
 
Offering Description Price**
Small Metagenome Minimum of 12M Illumina Reads (1Gbp) with Analysis $220.00
Large Metagenome Minimum of 25M Illumina Reads (2Gbp) with Analysis $340.00

*Average delivered amount is approximately 2-3 times higher than listed amounts
** Orders >48 Samples receive 10% off using Discount Code: Bulk10Savings

Sample Requirements

  • Volume of 30uL or more 
  • Sample concentration greater than 10ng/uL (measured by Qubit), or greater than 20ng/uL (measured by Nanodrop)*** of DNA
Additional Options
  • Genomic DNA isolation is available at a rate of $15 per sample.  We use Zymo Quick-DNA Fecal/Soil extractions which are compatible with a wide range of sample types.
***Please see our FAQ for more information about measurement methods.

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Not sure how your data sequencing can possibly keep up with your research? We're researchers first and businessmen second, so we are always working to meet your unique needs.
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