Dedicated Sequencing Runs

MiGS offers full flowcell packages for users that wish to run sequencing libraries that have been prepared outside of our labs.  Upon arrival, these pre-pooled libraries will be quanitifed in triplicate using High Sensitivity Qubit and analyzed for fragment size distribution using High Sensitivity DNA TapeStation reagents to calculate molarity and loaded onto the sequencer at the user-specified concentration.  If applicable, custom primers must be provided.  Data is delivered as a complete run folder and, if requested, demultiplexed folders of samples within two weeks of library arrival.

MiSeq Full Flowcells

It is highly recommended that library kit manufacturer recommendations be consulted to determine machine loading concentration.  We recommend loading concentrations of 8pM for v2 Flowcells and 10pM for v3 Flowcells when library kit manufacturers provide no other guidance.

Flowcell Type	Expected Data Output	Price
600cyc v3 Flowcell	25M SE Reads or 50M PE Reads, 15Gbs of Data	$2,100.00
500cyc v2 Flowcell	12.5M SE Reads or 25M PE Reads, 8Gbs of Data	$1,700.00
300cyc v2 Flowcell	12.5M SE Reads or 25M PE Reads, 4.8Gbs of Data	$1,500.00

 

NextSeq 550 Full Flowcells

We recommend loading concentrations of 1.5pM for Mid Output Flowcells and 1.7pM for High Output Flowcells when library kit manufacturers provide no other guidance.

Flowcell Type	Expected Data Output	Price
150cyc Mid Output	130M SE Reads or 260M PE Reads, 18Gbs of Data	$1,900.00
300cyc Mid Output	130M SE Reads or 260M PE Reads, 36Gbs of Data	$2,600.00
75cyc High Output	400M SE Reads or 800M PE Reads, 30Gbs of Data	$2,300.00
150cyc High Output	400M SE Reads or 800M PE Reads, 60Gbs of Data	$3,800.00
300cyc High Output	400M SE Reads or 800M PE Reads, 120Gbs of Data	$5,900.00