Using the most current set of primers available, MiGS will enrich your SARS-CoV-2 sample for viral reads using an tiled amplicon approach (based on Qiagen's v3 primers plus a booster set of primers to cover new variants). These amplicons are then prepared for sequencing using our standard whole genome sequencing pipeline with a target of 200Mbs of reads per sample. As part of this service, reads are assembled using the SARS-CoV-2 reference genome as a scaffold and both fastq and genome fasta files are provided as deliverables.
The ARTIC enrichment method has proven to be effective at generating >70% coverage across genomes for samples with Ct values as high as 35. Submitted samples are not measured for viral load before the library preparation process, so it is recommended that only samples with Ct values below 35 are used.
We are able to offer this service for any number of samples
at the below prices through our online ordering system
||Price per Sample
|VTM Media (requiring viral RNA extraction)
- Volume of 15uL or greater
- Must be shipped on dry ice